Cyclamen caulogenesis, rhizogenesis and microtuberization
Abstract
The tissue culture of Cyclamen persicum Mill. of the Primulaceae family, and a popular ornamental pot plant, is well explored. This synopsis review provides an overview of the induction of shoots (caulogenesis), roots (rhizogenesis) and microtubers (microtuberization) in vitro as viable routes for the tissue culture of clonal material, primarily from the English literature.
References
Abu-Qaoud H. 2004. Direct regeneration in Cyclamen persicum Mill. using seedling tissues. An-Najah Univ. J. Res. (Nat. Sci.) 18: 147–156.
Ando T., Murasaki K. 1983. In vitro propagation of Cyclamen by the use of etiolated petioles. Tech. Bull. Fac. Hort. Chiba Univ. 32: 1–5.
Bach A., Malik M., Zolneczko B. 1998. Organogenesis and embryogenesis in cultures of Cyclamen persicum Mill, F-1 ‘Medium’. Acta Biol. Cracov. Ser. Bot. 40: 47–51
Bourgin J.P., Nitsch J.P. 1967. Production of haploid Nicotiana from excised stamens. Ann. Physiol. Veg. 9: 377–382.
Bürün B., Şahin O. 2009. In vitro and in vivo germination of Cyclamen alpinum seeds. Turk. J. Bot. 33: 277–283. doi: 10.3906/bot-0709-8.
Dillen W., Dijkstra I., Oud J. 1996. Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. Plant Cell Reports 15: 545–548. doi: 10.1007/BF00232991.
Fukui H., Yamamoto T., Asano T., Nakamura M. 1988. Effect of plant growth regulators on in vitro organogenesis of cyclamen (Cyclamen persicum Mill.). Res. Bull. Faculty Agricult. Gifu Univ. 53: 139–145.
Gautherert J. 1954. Catalogue des cultures de tissus vegetaux. Rev. Gen. Bot. 61: 672–702.
Geier T. 1977. Morphogenesis and plant regeneration from cultured organ fragments of Cyclamen persicum. Acta Hortic. 78: 167–174. doi: 10.17660/ActaHortic.1977.78.20.
Hawkes H.Y., Wainwright H. 1987. In vitro organogenesis of Cyclamen persicum Mill. seedling tissue. Acta Hortic. 212: 711–714. doi: 10.17660/ActaHortic.1987.212.122.
Ishizaka H., Uematsu J. 1992. Production of interspecific hybrids of Cyclamen persicum Mill and C. hederifolium Aiton by ovule culture. Jpn. J. Breed. 42 (2): 354–366. doi: 10.1270/jsbbs1951.42.353.
Jalali N., Naderi R., Ali S.-G., Teixeira da Silva J.A. 2012. Tissue culture of Cyclamen spp. Sci. Hortic. 137: 11–19. doi: 10.1016/j.scienta.2012.01.015.
Karam N.S., Al-Majathoub M. 2000a. Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill. using seedling tissue. Sci. Hortic. 86: 235-246. doi: 10.1016/S0304-4238(00)00146-1.
Karam N.S., Al-Majathoub M. 200b. In vitro shoot regeneration from mature tissue of wild Cyclamen persicum Mill. Sci. Hortic. 86: 323–333. doi: 10.1016/S0304-4238(00)00160-6.
Linsmaier E.M., Skoog F. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127. doi: 10.1111/j.1399-3054.1965.tb06874.x.
Loewenberg J.R. 1969. Cyclamen callus culture. Can. J. Bot. 47 (12): 2065–2067. doi: 10.1139/b69-298
Mayer-Hörster L. 1956. Wachstum und Organbildung an in vitro kultivierten Segmenten von Pelargonium zonale und Cyclamen persicum. Planta 47: 401–446. doi: 10.1007/BF01911864.
Miller C.O. 1961. A kinetin like compound in maize. Proc. Nat. Acad. Sci. U.S.A. 47 (2): 170–174.
Mohannad A.M., Karam N.S. 2000. In vitro propagation of wild Cyclamen persicum Mill. from seedling tissue. Acta Hortic. 530: 243–252. doi: 10.17660/ActaHortic.2000.530.29.
Murasaki K., Tsurushima H. 1988. Improvement on clonal propagation of cyclamen in vitro by the use of etiolated petioles. Acta Hortic. 226: 721–724. doi: 10.17660/ActaHortic.1988.226.105.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497.
Nhut D.T., Thu H.T.M., Vinh B.V.T., Binh N.V., Luan V.Q. 2012. Thin cell layer technology in regeneration and micropropagation of Cyclamen persicum Mill. Propag. Ornam. Plants 12 (2): 89–95.
Nitsch J.P. 1951. Growth and development in vitro of excised ovaries. Amer. J. Bot. 38: 566–577.
Nitsch J.P. 1969. Experimental androgenesis in Nicotiana. Phytomorphol. 19: 389–404.
Nitsch J.P., Nitsch C. 1969. Haploid plants from pollen grains. Science 163 (3862): 85–89.
Okumoto H., Takabayashi S. 1969. Aseptic culture of cyclamen tuber tissue. J. Jpn. Soc. Hortic. Sci. 38 (2): 178–187. doi: 10.2503/jjshs.38.178.
Pierik R.L.M. 1975. Vegetatieve vermeerdering van Cyclamen. Vakblad voor Bloemisterij 30 (28): 13.
Prange A.N.S, Serek M., Winkelmann T. 2008. Vegetative propagation of different cyclamen species via adventitious shoot formation from seedling tissue. Propag. Ornam. Plants 8 (4): 204–209.
Schwenkel H.G., Grunewaldt J. 1988. In vitro propagation of Cyclamen persicum Mill. Acta Hortic. 226: 659–662. doi: 10.17660/ActaHortic.1988.226.92.
Seyring M., Ewald A., Mueller A., Haensch K.-T. 2009. Screening for propagation suitability in vitro of different Cyclamen species. Electron. J. Biotechnol. 12 (4). doi: 10.2225/vol12-issue4-fulltext-7.
Stichel E. 1959. Gleichzeitige Induktion von Sporossen und Wurzeln und in vitro Kultivierten Gewebestücken von Cyclamen persicum. Planta 53: 293–317. doi: 10.1007/BF01881794.
Tagipur E.M., Seker G., Teixeira da Silva J.A., Yelcin Mendi Y. 2016. Somatic embryogenesis, cryopreservation and in vitro mutagenesis in Cyclamen. In: Mujib A. (ed.), Somatic embryogenesis in ornamentals and its applications: 155–167. Springer, India. doi: 10.1007/978-81-322-2683-3_10.
Takamura T., Miyajima I., Maehara T. 1993. Seedling selection and micropropagation for the breeding of yellow-flowered Cyclamen cultivars. J. Fac. Agric. Kyushu Univ. 37 (3/4): 265–271.
Takamura T., Miyajima I. 1997. Micropropagation of Cyclamen persicum Mill. In: Bajaj Y.P.S (ed.), High‑tech and micropropagation VI. Biotechnology in Agriculture and Forestry 40: 96–112. Springer, Berlin. doi: 10.1007/978-3-662-03354-8_8.
Teixeira da Silva J.A. 2012. Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Australas. J. Plant Sci. Biotechnol. 6 (Special Issue 1): 121–124.
Teixeira da Silva J.A., Altamura M.M., Dobránszki J. 2015. The untapped potential of plant thin cell layers. J. Hortic. Res. 23 (2): 127–131. doi: 10.2478/johr-2015-0024.
Teixeira da Silva J.A., Dobránszki J. 2013a. How timing of sampling can affect the outcome of the quantitative assessment of plant organogenesis. Sci. Hortic. 159: 59–66. doi: 10.1016/j.scienta.2013.05.001.
Teixeira da Silva J.A., Dobránszki J. 2013b. Plant thin cell layers: a 40-year celebration. J. Plant Growth Regul. 32 (4): 922–943. doi: 10.1007/s00344-013-9336-6.
Teixeira da Silva J.A., Dobránszki J. 2014. Sonication and ultrasound: impact on plant growth and development. Plant Cell Tissue Organ Cult. 117 (2): 131–143. doi: 10.1007/s11240-014-0429-0.
Teixeira da Silva J.A., Dobránszki J. 2015a. Plant thin cell layers: update and perspectives. Folia Hort. 27 (2): 183–190. doi: 10.1515/fhort-2015-0029.
Teixeira da Silva J.A., Dobránszki J. 2015b. How do magnetic fields affect plants in vitro? In Vitro Cell Dev. Biol. Plant. 51 (3): 233–240. doi: 10.1007/s11627-015-9675-z.
Teixeira da Silva J.A., Kerbauy G.B., Zeng S-J., Chen Z-L., Duan J. 2014. In vitro flowering of orchids. Crit. Rev. Biotechnol. 34 (1): 56–76. doi: 10.3109/07388551.2013.807219.
Wainwright H., Harwood A.C. 1985. In vitro organogenesis and plant regeneration of Cyclamen persicum Mill. using seedling tissue. J. Hortic. Sci. 60: 397–403. doi: 10.1080/14620316.1985.11515644.
White P.R. 1963. The cultivation of animal and plant cells. Ronald Press, New York.
Wicart G., Mouras A., Lutz A. 1984. Histological study of organogenesis and embryogenesis in Cyclamen persicum Mill. tissue culture: evidence for a single organogenesis pattern. Protoplasma 119: 159–167. doi: 10.1007/BF01288870.
Yamaner Ö., Erdag B. 2008. Direct shoot formation and microtuberization from aseptic seedlings of Cyclamen mirabile Hildebr. Biotechnol. 7: 328–332. doi: 10.3923/biotech.2008.328.332.
Ando T., Murasaki K. 1983. In vitro propagation of Cyclamen by the use of etiolated petioles. Tech. Bull. Fac. Hort. Chiba Univ. 32: 1–5.
Bach A., Malik M., Zolneczko B. 1998. Organogenesis and embryogenesis in cultures of Cyclamen persicum Mill, F-1 ‘Medium’. Acta Biol. Cracov. Ser. Bot. 40: 47–51
Bourgin J.P., Nitsch J.P. 1967. Production of haploid Nicotiana from excised stamens. Ann. Physiol. Veg. 9: 377–382.
Bürün B., Şahin O. 2009. In vitro and in vivo germination of Cyclamen alpinum seeds. Turk. J. Bot. 33: 277–283. doi: 10.3906/bot-0709-8.
Dillen W., Dijkstra I., Oud J. 1996. Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill. Plant Cell Reports 15: 545–548. doi: 10.1007/BF00232991.
Fukui H., Yamamoto T., Asano T., Nakamura M. 1988. Effect of plant growth regulators on in vitro organogenesis of cyclamen (Cyclamen persicum Mill.). Res. Bull. Faculty Agricult. Gifu Univ. 53: 139–145.
Gautherert J. 1954. Catalogue des cultures de tissus vegetaux. Rev. Gen. Bot. 61: 672–702.
Geier T. 1977. Morphogenesis and plant regeneration from cultured organ fragments of Cyclamen persicum. Acta Hortic. 78: 167–174. doi: 10.17660/ActaHortic.1977.78.20.
Hawkes H.Y., Wainwright H. 1987. In vitro organogenesis of Cyclamen persicum Mill. seedling tissue. Acta Hortic. 212: 711–714. doi: 10.17660/ActaHortic.1987.212.122.
Ishizaka H., Uematsu J. 1992. Production of interspecific hybrids of Cyclamen persicum Mill and C. hederifolium Aiton by ovule culture. Jpn. J. Breed. 42 (2): 354–366. doi: 10.1270/jsbbs1951.42.353.
Jalali N., Naderi R., Ali S.-G., Teixeira da Silva J.A. 2012. Tissue culture of Cyclamen spp. Sci. Hortic. 137: 11–19. doi: 10.1016/j.scienta.2012.01.015.
Karam N.S., Al-Majathoub M. 2000a. Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill. using seedling tissue. Sci. Hortic. 86: 235-246. doi: 10.1016/S0304-4238(00)00146-1.
Karam N.S., Al-Majathoub M. 200b. In vitro shoot regeneration from mature tissue of wild Cyclamen persicum Mill. Sci. Hortic. 86: 323–333. doi: 10.1016/S0304-4238(00)00160-6.
Linsmaier E.M., Skoog F. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127. doi: 10.1111/j.1399-3054.1965.tb06874.x.
Loewenberg J.R. 1969. Cyclamen callus culture. Can. J. Bot. 47 (12): 2065–2067. doi: 10.1139/b69-298
Mayer-Hörster L. 1956. Wachstum und Organbildung an in vitro kultivierten Segmenten von Pelargonium zonale und Cyclamen persicum. Planta 47: 401–446. doi: 10.1007/BF01911864.
Miller C.O. 1961. A kinetin like compound in maize. Proc. Nat. Acad. Sci. U.S.A. 47 (2): 170–174.
Mohannad A.M., Karam N.S. 2000. In vitro propagation of wild Cyclamen persicum Mill. from seedling tissue. Acta Hortic. 530: 243–252. doi: 10.17660/ActaHortic.2000.530.29.
Murasaki K., Tsurushima H. 1988. Improvement on clonal propagation of cyclamen in vitro by the use of etiolated petioles. Acta Hortic. 226: 721–724. doi: 10.17660/ActaHortic.1988.226.105.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497.
Nhut D.T., Thu H.T.M., Vinh B.V.T., Binh N.V., Luan V.Q. 2012. Thin cell layer technology in regeneration and micropropagation of Cyclamen persicum Mill. Propag. Ornam. Plants 12 (2): 89–95.
Nitsch J.P. 1951. Growth and development in vitro of excised ovaries. Amer. J. Bot. 38: 566–577.
Nitsch J.P. 1969. Experimental androgenesis in Nicotiana. Phytomorphol. 19: 389–404.
Nitsch J.P., Nitsch C. 1969. Haploid plants from pollen grains. Science 163 (3862): 85–89.
Okumoto H., Takabayashi S. 1969. Aseptic culture of cyclamen tuber tissue. J. Jpn. Soc. Hortic. Sci. 38 (2): 178–187. doi: 10.2503/jjshs.38.178.
Pierik R.L.M. 1975. Vegetatieve vermeerdering van Cyclamen. Vakblad voor Bloemisterij 30 (28): 13.
Prange A.N.S, Serek M., Winkelmann T. 2008. Vegetative propagation of different cyclamen species via adventitious shoot formation from seedling tissue. Propag. Ornam. Plants 8 (4): 204–209.
Schwenkel H.G., Grunewaldt J. 1988. In vitro propagation of Cyclamen persicum Mill. Acta Hortic. 226: 659–662. doi: 10.17660/ActaHortic.1988.226.92.
Seyring M., Ewald A., Mueller A., Haensch K.-T. 2009. Screening for propagation suitability in vitro of different Cyclamen species. Electron. J. Biotechnol. 12 (4). doi: 10.2225/vol12-issue4-fulltext-7.
Stichel E. 1959. Gleichzeitige Induktion von Sporossen und Wurzeln und in vitro Kultivierten Gewebestücken von Cyclamen persicum. Planta 53: 293–317. doi: 10.1007/BF01881794.
Tagipur E.M., Seker G., Teixeira da Silva J.A., Yelcin Mendi Y. 2016. Somatic embryogenesis, cryopreservation and in vitro mutagenesis in Cyclamen. In: Mujib A. (ed.), Somatic embryogenesis in ornamentals and its applications: 155–167. Springer, India. doi: 10.1007/978-81-322-2683-3_10.
Takamura T., Miyajima I., Maehara T. 1993. Seedling selection and micropropagation for the breeding of yellow-flowered Cyclamen cultivars. J. Fac. Agric. Kyushu Univ. 37 (3/4): 265–271.
Takamura T., Miyajima I. 1997. Micropropagation of Cyclamen persicum Mill. In: Bajaj Y.P.S (ed.), High‑tech and micropropagation VI. Biotechnology in Agriculture and Forestry 40: 96–112. Springer, Berlin. doi: 10.1007/978-3-662-03354-8_8.
Teixeira da Silva J.A. 2012. Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Australas. J. Plant Sci. Biotechnol. 6 (Special Issue 1): 121–124.
Teixeira da Silva J.A., Altamura M.M., Dobránszki J. 2015. The untapped potential of plant thin cell layers. J. Hortic. Res. 23 (2): 127–131. doi: 10.2478/johr-2015-0024.
Teixeira da Silva J.A., Dobránszki J. 2013a. How timing of sampling can affect the outcome of the quantitative assessment of plant organogenesis. Sci. Hortic. 159: 59–66. doi: 10.1016/j.scienta.2013.05.001.
Teixeira da Silva J.A., Dobránszki J. 2013b. Plant thin cell layers: a 40-year celebration. J. Plant Growth Regul. 32 (4): 922–943. doi: 10.1007/s00344-013-9336-6.
Teixeira da Silva J.A., Dobránszki J. 2014. Sonication and ultrasound: impact on plant growth and development. Plant Cell Tissue Organ Cult. 117 (2): 131–143. doi: 10.1007/s11240-014-0429-0.
Teixeira da Silva J.A., Dobránszki J. 2015a. Plant thin cell layers: update and perspectives. Folia Hort. 27 (2): 183–190. doi: 10.1515/fhort-2015-0029.
Teixeira da Silva J.A., Dobránszki J. 2015b. How do magnetic fields affect plants in vitro? In Vitro Cell Dev. Biol. Plant. 51 (3): 233–240. doi: 10.1007/s11627-015-9675-z.
Teixeira da Silva J.A., Kerbauy G.B., Zeng S-J., Chen Z-L., Duan J. 2014. In vitro flowering of orchids. Crit. Rev. Biotechnol. 34 (1): 56–76. doi: 10.3109/07388551.2013.807219.
Wainwright H., Harwood A.C. 1985. In vitro organogenesis and plant regeneration of Cyclamen persicum Mill. using seedling tissue. J. Hortic. Sci. 60: 397–403. doi: 10.1080/14620316.1985.11515644.
White P.R. 1963. The cultivation of animal and plant cells. Ronald Press, New York.
Wicart G., Mouras A., Lutz A. 1984. Histological study of organogenesis and embryogenesis in Cyclamen persicum Mill. tissue culture: evidence for a single organogenesis pattern. Protoplasma 119: 159–167. doi: 10.1007/BF01288870.
Yamaner Ö., Erdag B. 2008. Direct shoot formation and microtuberization from aseptic seedlings of Cyclamen mirabile Hildebr. Biotechnol. 7: 328–332. doi: 10.3923/biotech.2008.328.332.
Published
2016-06-01
How to Cite
TEIXEIRA DA SILVA, Jaime A..
Cyclamen caulogenesis, rhizogenesis and microtuberization.
Modern Phytomorphology, [S.l.], v. 10, p. 3-10, june 2016.
ISSN 2227-9555.
Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/8>. Date accessed: 11 apr. 2018.
doi: https://doi.org/10.5281/zenodo.155344.
Issue
Section
Review Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The journal is licensed by Creative Commons under BY-NC-ND license. You are welcome and free to share (copy and redistribute the material in any medium or format) all the published materials. You may not use the material for commercial purposes. You must give appropriate credit to all published materials.
The journal allow the author(s) to hold the copyrights and to retain publishing rights without any restrictions. This is also indicated at the bottom of each article.
