Trichomes micromorphology and cytological investigation on Acinos alpinus subsp. meridionalis in Algeria
Abstract
Anatomical and karyological features were investigated in four populations of Acinos alpinus subsp. meridionalis (Nyman) P. Ball., an Ibero-Maghreban species. Plant material was collected in the field during flowering stage, in contrasting bioclimatic conditions. Fresh material was used for analysis of trichomes morphology with light microscopy. Young meristems and flower buds were fixed for karyological analysis. Two types of capitate glandular, one type of peltate glandular, and four types of non-glandular trichomes were observed on different parts of the plants, including the stems, leaves and flowers. Trichomes distribution and density showed variability among organs, but no intraspecific variability was found. The chromosome number 2n = 2x = 18 is reported for the first time for Algerian populations. Furthermore, aberrant meiotic behavior in the form of cytomixis was observed in one population of A. alpinus subsp. meridionalis – cytoplasmic strand was formed between more than two cells at different stages of meiosis. Other types of abnormalities such as lagging chromosomes, and formation of triads with microcytes were also discovered. Results are discussed in comparison with literature.
References
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Ball P.W. 1972. Taxonomic and nomenclatural notes on European Labiatae (Acinos Miller, Calamintha Miller, Lamium L., Satureja L.). In: Heywood V.H. (ed.), Flora Europaea: Notulae Systematicae ad Floram Europaeam spectantes: No. 13. Bot. J. Linn. Soc. 65 (4): 342–352. https://doi.org/10.1111/j.1095-8339.1972.tb02277.x
Battandier J.A., Trabut L. 1902. Flore analytique et synoptique de l'Algérie et de la Tunisie: 256–260. Ed. Vve Giralt, Alger.
Bellucci M., Roscini C., Mariani A. 2003. Cytomixis in pollen mother cells of Medicago sativa L. J. Hered. 94 (6): 512–516. https://doi.org/10.1093/jhered/esg096
Bezić N., Dunkić V., Radonić A. 2001. Glandular appartus structure and essential oil constituents of Satureja cuneifolia Ten. Acta Biol. Cracov. Ser. Bot. 43: 65–68. https://doi.org/10.1002/ptr.1290
Cantino P.D. 1990. The phylogentic significance of stomata and trichomes in the Labiatae and Verbenaceae. J. Arnold Arbor. 71 (3): 323–370. http://www.jstor.org/stable/43782250
Datta A.K., Mukherjee M., Iqbal M. 2005. Persistent cytomixis in Ocimum basilicum L. (Lamiaceae) and Withania somnifera (L.) Dun (Solanaceae). Cytol. 70: 309–313. https://doi.org/10.1508/cytologia.70.309
de Montmollin B. 1986. Étude cytotaxonomique de la flore de la Crète. III. Nombres chromosomiques. Candollea 41: 431–439.
Deysson G. 1954. Eléments d’anatomie des plantes vasculaires. Sedes, Paris.
Dobignard A., Chatelain C. 2012. Index synonymique de la flore d’Afrique du Nord. Vol. 4: 260–318. Dicotyledoneae: Fabaceae – Nymphaceae. Ed. Conservatoires et Jardin Botaniques, Genève.
Fahn A. 1988. Secretory tissues in vascular plants. New Phytol. 108 (3): 229–257. https://doi.org/10.1111/j.1469-8137.1988.tb04159.x
Ferandes R. 1959. As plantas portuguesas da secção Acinos (Moench) Briquet do género Satureja L. Boletim da Sociedade Broteriana, Série 2 33: 120–143.
Galland N. 1988. Recherche sur l'origine de la flore orophile du Maroc étude caryologique et cytogéographique. Trav. Inst. Sci. Univ. Mohammed V, Sér. Bot. 35: 1–168.
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Harley R.M., Atkins S., Budantsev A.L., Cantino P.D., Conn B.J., Grayer R., Harley M.M., de Kok R., Krestovskaja T., Morales R., Paton A.J., Ryding O., Upson T. 2004. Labiatae. In: Kadereit J.W. (ed.), Flowering Plants. Dicotyledons: 167–275. Springer-Verlag Berlin, Heidelberg.
Jahier J., Chevre A.M., Delourme R., Eber F., Tanguy A.M. 1992. Techniques de cytogénétique végétale: 22–36. Ed. INRA.
Kandemir N. 2003. The morphological, anatomical and karyological properties of endemic Salvia hypargela Fich. and Mey. (Lamiaceae) in Turkey. Pak. J. Bot. 35 (2): 219–236.
Kaya A. 2016. Comparative root and stem anatomy of six Clinopodium (Lamiaceae) taxa. Biologia 71/12: 1330–1337. https://doi.org/10.1515/biolog-2016-0163
Kaya A., Koca F. 2004. Comparative leaf anatomical studies of Acinos species (Labiatae) from Turkey. Nord. J. Bot. 23 (5): 1–12. https://doi.org/10.1111/j.1756-1051.2003.tb00437.x
Khaled-Khodja N., Boulekbache-Makhlouf L., Madani K. 2014. Phytochemical screening of antioxidant and antibacterial activities of methanolic extracts of some Lamiaceae. Ind. Crops Prod. 61: 41–48. https://doi.org/10.1016/j.indcrop.2014.06.037
Mandal A., Datta A.K., Ghosh B.K., Gupta S., Paul R., Saha A., Ghosh B.K., Bhattacharya A., Iqbal M. 2013. Cytomixis a unique phenomenon in animal and plant. Protoplasma 250: 985–996. https://doi.org/10.1007/s00709-013-0493-z
Marin M., Koko V., Duletič-Lausevic S., Marin P.D. 2008. Micromorphology of trichomes of Thymus malyi (Lamiaceae). J. Microsc. 232 (3): 406–409. https://doi.org/10.1111/j.1365-2818.2008.02135.x
Mártonfi P., Mártonfiová L. 1996. Thymus chromosome numbers from Carpathians and Pannonia. Thaisia, J. Bot. Košice 6: 25–38.
Miller P. 1754. The gardeners dictionary: Containing the methods of cultivating and improving all sorts of trees, plants, and flowers, for the kitchen, fruit, and pleasure gardens. John and James Rivington, London.
Mondolot L., Roussel J.L., Andary C. 2001. New applications for an old lignified element staining reagent. Histochem J. 33: 379–385. https://doi.org/10.1023/A:1013798426161
Moon H.-K., Hong S.-P., Smets E., Huysmans S. 2009. Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Bot. J. Linn. Soc. 160: 211–231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
Moon H.-K., Smets E., Huysmans S. 2010. Phylogeny of tribe Mentheae (Lamiaceae): The story of molecules and micromorphological characters. Taxon 59 (4): 1065–1076. https://doi.org/10.2307/20773977
Morales R. 1980. Números cromosomáticos en especies ibéricas del género Thymus L. (Labiatae). An. Jard. Bot. Madrid 36 (1): 339–348.
Morales R. 1986. Notas citotaxonómicas sobre algunes tomillos ibéricos y norte africanos (Thymus L. Labiatae). An. Jard. Bot. Madrid 43 (1): 35–41.
Morales R. 1990. Números cromosomáticos de plantas occidentales, 582–590. An. Jard. Bot. Madrid 47 (1): 193–198.
Morales R., Quintanar A., Cabezas F., Pujadas A.J., Cirujano S. 2010. Flora Ibérica. Plantas vasculares de la península Ibérica e islas Baleares. Vol. XII. Verbenaceae – Labiatae – Callitrichaceae. Real Jardín Botánico, C.S.I.C. Madrid. http://www.floraiberica.es/miscelania/noticias/Volumen_XII.php
Naidoo Y., Kasim N., Heneidak S., Nicholas A., Naidoo G. 2013. Foliar secretory trichomes of Ocimum obovatum (Lamiaceae): Micromorphological structure and histochemistry. Plant Syst. Evol. 299: 873–885. https://doi.org/10.1007/s00606-013-0770-5
Navarro T., El Oualidi J. 2000. Morfología de los tricomas en Teucrium L. (Labiatae). Una revisíon taxonómica. An. Jard. Bot. Madrid 57 (2): 277–297. https://doi.org/10.3989/ajbm.1999.v57.i2.203
Öztürk Çalı İ. 2017.Glandular trichomes on vegetative and reproductive organs of Lamium orientale (Lamiaceae). Mod. Phytomorphol. 11: 15–19. https://doi.org/10.5281/zenodo.398865
Quezel P., Santa S. 1963. Nouvelle flore de l’Algérie et régions désertiques méridionales. T. II: 781–793. Ed. C.N.R.S., Paris.
Ruíz de Clavijo E. 1993. Números cromosomáticos para la flora Española, 664–690. Lagascalia 17: 161–172.
Salmaki Y., Zarre S., Jamzad Z., Bräuchler C. 2009. Trichome micromorphology of Iranian Stachys (Lamiaceae) with emphasis on its systematic implication. Flora 204 (5): 371–381. https://doi.org/10.1016/j.flora.2008.11.001
Song Z.Q., Li X.F. 2009. Cytomixis in pollen mother cells of Salvia miltiorrhiza. Caryologia 62: 213–219. https://doi.org/10.1080/00087114.2004.10589687
Strid A. 1983. Acinos alpinus subsp. meridionalis (Nyman) P.W. Ball. In: Löve A. (ed.), IOPB chromosome number reports LXXVIII. Taxon 32: 138–140.
Ubera J.L. 1979. 104. Acinos alpinus subsp. meridionalis (Nyman) P.W. Ball. In: Números cromosómicos para la flora Española. 84–120. Lagascalia 9 (1): 123–124.
Werker E., Putievsky E., Ravid U., Dudai N., Katzir L. 1993. Glandular hairs and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Ann. Bot. 71: 43–50. https://doi.org/10.1006/anbo.1993.1005
Antunes T., Sevinate-Pinto I. 1991. Glandular trichomes of Teucrium scorodonia L. Morphology and histochemistery. Flora 185 (1): 65–70. https://doi.org/10.1016/S0367-2530(17)30445-0
Antunes T., Sevinate-Pinto I., Fagueiredo A.C., Barroso J.G., Pedro L.G., Fontinha S.S., Scheffer J.C. 1997. Morphology and distribution of trichomes in two endemic Teucrium species of Macaronesia. Acta Bot. Gall. 144 (3): 363–369. https://doi.org/10.1080/12538078.1997.10515381
Ascensão L., Pais M.S. 1998. The leaf capitate trichomes of Leonitis leonurus: Histochemistry, ultrastructure and secretion. Ann. Bot. 81 (2): 263–271. https://doi.org/10.1006/anbo.1997.0550
Ball P.W. 1972. Taxonomic and nomenclatural notes on European Labiatae (Acinos Miller, Calamintha Miller, Lamium L., Satureja L.). In: Heywood V.H. (ed.), Flora Europaea: Notulae Systematicae ad Floram Europaeam spectantes: No. 13. Bot. J. Linn. Soc. 65 (4): 342–352. https://doi.org/10.1111/j.1095-8339.1972.tb02277.x
Battandier J.A., Trabut L. 1902. Flore analytique et synoptique de l'Algérie et de la Tunisie: 256–260. Ed. Vve Giralt, Alger.
Bellucci M., Roscini C., Mariani A. 2003. Cytomixis in pollen mother cells of Medicago sativa L. J. Hered. 94 (6): 512–516. https://doi.org/10.1093/jhered/esg096
Bezić N., Dunkić V., Radonić A. 2001. Glandular appartus structure and essential oil constituents of Satureja cuneifolia Ten. Acta Biol. Cracov. Ser. Bot. 43: 65–68. https://doi.org/10.1002/ptr.1290
Cantino P.D. 1990. The phylogentic significance of stomata and trichomes in the Labiatae and Verbenaceae. J. Arnold Arbor. 71 (3): 323–370. http://www.jstor.org/stable/43782250
Datta A.K., Mukherjee M., Iqbal M. 2005. Persistent cytomixis in Ocimum basilicum L. (Lamiaceae) and Withania somnifera (L.) Dun (Solanaceae). Cytol. 70: 309–313. https://doi.org/10.1508/cytologia.70.309
de Montmollin B. 1986. Étude cytotaxonomique de la flore de la Crète. III. Nombres chromosomiques. Candollea 41: 431–439.
Deysson G. 1954. Eléments d’anatomie des plantes vasculaires. Sedes, Paris.
Dobignard A., Chatelain C. 2012. Index synonymique de la flore d’Afrique du Nord. Vol. 4: 260–318. Dicotyledoneae: Fabaceae – Nymphaceae. Ed. Conservatoires et Jardin Botaniques, Genève.
Fahn A. 1988. Secretory tissues in vascular plants. New Phytol. 108 (3): 229–257. https://doi.org/10.1111/j.1469-8137.1988.tb04159.x
Ferandes R. 1959. As plantas portuguesas da secção Acinos (Moench) Briquet do género Satureja L. Boletim da Sociedade Broteriana, Série 2 33: 120–143.
Galland N. 1988. Recherche sur l'origine de la flore orophile du Maroc étude caryologique et cytogéographique. Trav. Inst. Sci. Univ. Mohammed V, Sér. Bot. 35: 1–168.
Govaerts R.H.A. 1999. World checklist of seed plants. Vol. 3, Part 1: 16. Continental Publishing, Antwerp.
Harley R.M., Atkins S., Budantsev A.L., Cantino P.D., Conn B.J., Grayer R., Harley M.M., de Kok R., Krestovskaja T., Morales R., Paton A.J., Ryding O., Upson T. 2004. Labiatae. In: Kadereit J.W. (ed.), Flowering Plants. Dicotyledons: 167–275. Springer-Verlag Berlin, Heidelberg.
Jahier J., Chevre A.M., Delourme R., Eber F., Tanguy A.M. 1992. Techniques de cytogénétique végétale: 22–36. Ed. INRA.
Kandemir N. 2003. The morphological, anatomical and karyological properties of endemic Salvia hypargela Fich. and Mey. (Lamiaceae) in Turkey. Pak. J. Bot. 35 (2): 219–236.
Kaya A. 2016. Comparative root and stem anatomy of six Clinopodium (Lamiaceae) taxa. Biologia 71/12: 1330–1337. https://doi.org/10.1515/biolog-2016-0163
Kaya A., Koca F. 2004. Comparative leaf anatomical studies of Acinos species (Labiatae) from Turkey. Nord. J. Bot. 23 (5): 1–12. https://doi.org/10.1111/j.1756-1051.2003.tb00437.x
Khaled-Khodja N., Boulekbache-Makhlouf L., Madani K. 2014. Phytochemical screening of antioxidant and antibacterial activities of methanolic extracts of some Lamiaceae. Ind. Crops Prod. 61: 41–48. https://doi.org/10.1016/j.indcrop.2014.06.037
Mandal A., Datta A.K., Ghosh B.K., Gupta S., Paul R., Saha A., Ghosh B.K., Bhattacharya A., Iqbal M. 2013. Cytomixis a unique phenomenon in animal and plant. Protoplasma 250: 985–996. https://doi.org/10.1007/s00709-013-0493-z
Marin M., Koko V., Duletič-Lausevic S., Marin P.D. 2008. Micromorphology of trichomes of Thymus malyi (Lamiaceae). J. Microsc. 232 (3): 406–409. https://doi.org/10.1111/j.1365-2818.2008.02135.x
Mártonfi P., Mártonfiová L. 1996. Thymus chromosome numbers from Carpathians and Pannonia. Thaisia, J. Bot. Košice 6: 25–38.
Miller P. 1754. The gardeners dictionary: Containing the methods of cultivating and improving all sorts of trees, plants, and flowers, for the kitchen, fruit, and pleasure gardens. John and James Rivington, London.
Mondolot L., Roussel J.L., Andary C. 2001. New applications for an old lignified element staining reagent. Histochem J. 33: 379–385. https://doi.org/10.1023/A:1013798426161
Moon H.-K., Hong S.-P., Smets E., Huysmans S. 2009. Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Bot. J. Linn. Soc. 160: 211–231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
Moon H.-K., Smets E., Huysmans S. 2010. Phylogeny of tribe Mentheae (Lamiaceae): The story of molecules and micromorphological characters. Taxon 59 (4): 1065–1076. https://doi.org/10.2307/20773977
Morales R. 1980. Números cromosomáticos en especies ibéricas del género Thymus L. (Labiatae). An. Jard. Bot. Madrid 36 (1): 339–348.
Morales R. 1986. Notas citotaxonómicas sobre algunes tomillos ibéricos y norte africanos (Thymus L. Labiatae). An. Jard. Bot. Madrid 43 (1): 35–41.
Morales R. 1990. Números cromosomáticos de plantas occidentales, 582–590. An. Jard. Bot. Madrid 47 (1): 193–198.
Morales R., Quintanar A., Cabezas F., Pujadas A.J., Cirujano S. 2010. Flora Ibérica. Plantas vasculares de la península Ibérica e islas Baleares. Vol. XII. Verbenaceae – Labiatae – Callitrichaceae. Real Jardín Botánico, C.S.I.C. Madrid. http://www.floraiberica.es/miscelania/noticias/Volumen_XII.php
Naidoo Y., Kasim N., Heneidak S., Nicholas A., Naidoo G. 2013. Foliar secretory trichomes of Ocimum obovatum (Lamiaceae): Micromorphological structure and histochemistry. Plant Syst. Evol. 299: 873–885. https://doi.org/10.1007/s00606-013-0770-5
Navarro T., El Oualidi J. 2000. Morfología de los tricomas en Teucrium L. (Labiatae). Una revisíon taxonómica. An. Jard. Bot. Madrid 57 (2): 277–297. https://doi.org/10.3989/ajbm.1999.v57.i2.203
Öztürk Çalı İ. 2017.Glandular trichomes on vegetative and reproductive organs of Lamium orientale (Lamiaceae). Mod. Phytomorphol. 11: 15–19. https://doi.org/10.5281/zenodo.398865
Quezel P., Santa S. 1963. Nouvelle flore de l’Algérie et régions désertiques méridionales. T. II: 781–793. Ed. C.N.R.S., Paris.
Ruíz de Clavijo E. 1993. Números cromosomáticos para la flora Española, 664–690. Lagascalia 17: 161–172.
Salmaki Y., Zarre S., Jamzad Z., Bräuchler C. 2009. Trichome micromorphology of Iranian Stachys (Lamiaceae) with emphasis on its systematic implication. Flora 204 (5): 371–381. https://doi.org/10.1016/j.flora.2008.11.001
Song Z.Q., Li X.F. 2009. Cytomixis in pollen mother cells of Salvia miltiorrhiza. Caryologia 62: 213–219. https://doi.org/10.1080/00087114.2004.10589687
Strid A. 1983. Acinos alpinus subsp. meridionalis (Nyman) P.W. Ball. In: Löve A. (ed.), IOPB chromosome number reports LXXVIII. Taxon 32: 138–140.
Ubera J.L. 1979. 104. Acinos alpinus subsp. meridionalis (Nyman) P.W. Ball. In: Números cromosómicos para la flora Española. 84–120. Lagascalia 9 (1): 123–124.
Werker E., Putievsky E., Ravid U., Dudai N., Katzir L. 1993. Glandular hairs and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Ann. Bot. 71: 43–50. https://doi.org/10.1006/anbo.1993.1005
Published
2018-04-10
How to Cite
SAOULI, Nacira et al.
Trichomes micromorphology and cytological investigation on Acinos alpinus subsp. meridionalis in Algeria.
Modern Phytomorphology, [S.l.], v. 12, p. 33-42, apr. 2018.
ISSN 2227-9555.
Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/517>. Date accessed: 08 sep. 2018.
doi: https://doi.org/10.5281/zenodo.1216175.
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Research Articles
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