The influence of medium solidifying agents and double-phase medium on the growth and development of Cosmos atrosanguineus (Hook.) Voss in vitro

  • Danuta Kozak Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego str., 20-068 Lublin, Poland
  • Marzena Parzymies Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego str., 20-068 Lublin, Poland
  • Marek Dąbski Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego str., 20-068 Lublin, Poland
DOI: https://doi.org/10.5281/zenodo.161990

Abstract

Influence of different medium gelling agents (Agar-Agar Sigma, Lab-AgarTM Biocorp, Bacto-Agar Difco, Gelrite) and two types of medium (solidified by Agar-Agar and double-phase medium) on branching and growth of Cosmos atrosanguineus shoots was investigated. Shoot tips obtained from aseptical tissue cultures were grown for 6 weeks on Murashige and Skoog medium containing BA in concentration of 1 mg ∙ dm-3. It was found that induction of axillary shoots was the best on double-phase medium (addition of liquid medium after 4 weeks of shoot cultures in vitro). No significant differences were found in regeneration potential and elongation of shoots depending of the media solidified by studied agars or Gelrite.

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Published
2013-04-01
How to Cite
KOZAK, Danuta; PARZYMIES, Marzena; DĄBSKI, Marek. The influence of medium solidifying agents and double-phase medium on the growth and development of Cosmos atrosanguineus (Hook.) Voss in vitro. Modern Phytomorphology, [S.l.], v. 3, p. 73-75, apr. 2013. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/331>. Date accessed: 11 apr. 2018. doi: https://doi.org/10.5281/zenodo.161990.
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Vol 3 (2013)
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Correspondences
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