Effect of estrone on somatic and female gametophyte cell division and differentiation in Arabidospis thaliana cultured in vitro

  • Piotr Żabicki Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland
  • Joanna Rojek Department of Plant Cytology and Embryology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland
  • Małgorzata Kapusta Department of Plant Cytology and Embryology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland
  • Elżbieta Kuta Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland
  • Jerzy Bohdanowicz Department of Plant Cytology and Embryology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland
DOI: https://doi.org/10.5281/zenodo.160997

Abstract

The aim of the study was to determine the effect of the mammalian female sex hormone estrone on differentiation of somatic tissues and on induction of autonomous endosperm in culture of female gametophyte cells of Arabidopsis thaliana ecotype Columbia (Col-0).
In culture, estrone-stimulated development of autonomous endosperm (AE) occurred in 14.7% of unpollinated pistils. The AE represented development stages similar to those of young endosperm after fertilization and AE of fis mutants in vivo. In the majority of ovules the AE was in a few-nucleate young stage. Some ovules showed more advanced stages of AE development, with nuclei and cytoplasm forming characteristic nuclear cytoplasmic domains (NCDs). Sporadically, AE was divided into regions characteristic for Arabidopsis endosperm formed after fertilization.
Direct organogenesis (caulogenesis, rhizogenesis), callus proliferation and formation of trichome-like structures were observed during in vitro culture of hypocotyls and cotyledons of 3-day-old seedlings cultured on medium supplemented with estrone for 28 days. Histological analysis showed adventitious root formation and changes in explant anatomy caused by estrone.

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◀ Fig. 1. Unpollinated Arabidopsis Col-0 pistils at inoculation and cultured on MS 6% + estrone 1 µM for 7 days (a-d), and longitudinal paraffin sections of ovules inside unpollinated ovaries (e-g): a, b – pistils at inoculation; enlarged pistils (c) and ovules inside ovaries (d) after 7 days of culture; e – AE with two distant nuclei (arrows); f, g – few nucleate AE (arrows) accompanied by egg apparatus, visible synergids (sc). Bar – 1 mm (a-d) and 10 µm (e-g).
Published
2014-04-01
How to Cite
ŻABICKI, Piotr et al. Effect of estrone on somatic and female gametophyte cell division and differentiation in Arabidospis thaliana cultured in vitro. Modern Phytomorphology, [S.l.], v. 5, p. 25-30, apr. 2014. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/188>. Date accessed: 11 apr. 2018. doi: https://doi.org/10.5281/zenodo.160997.
Citation Formats
Issue
Vol 5 (2014)
Section
Research Articles
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