The features of shoots regeneration of European forsythia (Forsythia europaea Degen et Bald.) in sterile culture

  • Andrei V. Konstantinov Forest Research Institute, NAS of Belarus, Proletarian str. 71, 246001 Gomel, Belarus
DOI: https://doi.org/10.5281/zenodo.160768

Abstract

The effects of several factors such as medium, the source of cutting material and plant growth regulators concentration on in vitro culture and rapid propagation of Forsythia europaea were investigated. The buds from middle part of shoots sprouted in spring were the optimum type of explants, because of the highest shoot growth rate during initiation procedure and biggest amount of formed shoots per microcutting during subsequent multiplication. The best medium during initiation was WPM supplemented with 0.5 mg·l-1 6-BAP and 0.5 mg·l-1 NAA. Multiplication coefficient was up to 4.8±0,9 on MS medium supplemented with 0.5 mg·l-1 6-BAP at. Shoots were rooted on the WPM medium without growth regulators and the rate of rooting was 71-84%. The microplants obtained transferred to acclimatization substrate (mixture of peat, sand and perlite in proportions 3:1:2) with up to 96% survival.

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Fig. 1. Microshoots regeneration on the explants isolated from different parts of the green cutting: a – basal part (adventitious shoot present); б – apical part; в – middle part.
Published
2014-04-01
How to Cite
KONSTANTINOV, Andrei V.. The features of shoots regeneration of European forsythia (Forsythia europaea Degen et Bald.) in sterile culture. Modern Phytomorphology, [S.l.], v. 6, p. 225-230, apr. 2014. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/158>. Date accessed: 11 apr. 2018. doi: https://doi.org/10.5281/zenodo.160768.
Citation Formats
Issue
Vol 6 (2014)
Section
Research Articles
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