In vitro organogenesis in Rumex thyrsiflorus Fingerh. – problems of sex ratios
Abstract
Rumex thyrsiflorus Fingerh. is one of the few dioecious plant species, which have sex chromosomes. We conducted the preliminary experiments to determine the type of morphogenesis of R. thyrsiflorus explants cultured in vitro and to verify, using PCR-based methods, if there is the relationship between sex and morphogenetic response of explants micropropagated under in vitro conditions. The results of our studies revealed the female-biased sex ratios among explants cultured in vitro (M:F=1:1.7). The female-biased sex ratios in case of explants showed organogenesis in vitro (M:F=1:2.44) may suggest a higher regeneration ability of female explants.
References
Gawal N.J., Jarret R.L. 1991. A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol. Biol. Rep. 9: 262–266.
Grabowska-Joachimiak A., Kwolek D., Kula A., Marciniuk P. 2012. Fluorescent banding pattern and species-specific DNA marker in Rumex thyrsiflorus Fingerh. Cytogenet. Genome Res. 137: 70–77.
Korpelainen H. 2002. A genetic method to resolve gender complements investigations on sex ratios in Rumex acetosa. Mol. Ecol. 11: 2151–2156.
Kwolek D., Joachimiak A.J. 2011. Seed sexing revealed female bias in two Rumex species. Acta Soc. Bot. Pol. 80 (2): 93–97.
Mariotti B., Manzano S., Kejnovsky E., Vyskot B., Jamilena M. 2009. Accumulation of Y-specific satellite DNAs during the evolution of Rumex acetosa sex chromosomes. Mol. Genet. Genomics 281: 249–259.
Navajas-Peréz R., De La Herran R., Jamilena M., Lozano R., Ruiz Rejón M., Garrido-Ramos M.A. 2005. Reduced rates of sequence evolution of Y-linked satellite DNA in Rumex (Polygonaceae). J. Mol. Evol. 60: 391–399.
Navajas-Peréz R., Schwarzacher T., De La Herran R., Ruiz Rejón C., Ruiz Rejón M., Garrido-Ramos M.A. 2006. The origin and evolution of the variability in a Y-specific satellite-DNA of Rumex acetosa and its relatives. Gene 368: 61–71.
Rychlewski J., Zarzycki K. 1986. Genetical and ecological mechanisms regulating the sex ratio in populations of Rumex thyrsiflorus Fingerh. (Polygonaceae). Bull. Geobot. Inst. ETH 87: 132–140.
Ślesak H., Góralski G., Pawłowska H., Skucińska B., Popielarska-Konieczna M., Joachimiak A.J. 2013. The effect of genotype on a barley scutella culture. Histological aspects. Cent. Eur. J. Biol. 8 (1): 30–37.
Żuk J. 1963. An investigation on polyploidy and sex-determination within the genus Rumex. Acta Soc. Bot. Pol. 32: 5–72.
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